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Honors Molecular Biology

In Molecular Biology, students do numerous labs including: gel electrophoresis, polymerase chain reaction, bacterial transformation, column chromatography, ELISAs, plant tissue cultures, microarrays, cell culture, and media prep. The seniors also focus on recombinant DNA technology and examine pGLO plasmids.

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Ms. Wagner

Course  Glimpse

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Biotech Seniors used PCR (Polymerase Chain Reaction) to amplify target sequences of a patient's DNA. Following this procedure, the results were visualized through gel electrophoresis to detect COVID-19.
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(Top image) Seniors had purified GFP (Green Fluorescent Protein) from their bacterial transformation. 




(Bottom Image) Here are the results of the seniors' bacterial transformation lab.
(Top Image) Seniors run their vertical gel electrophoresis labs but instead of using agarose gel, acrylamide is preferred when dealing with smaller DNA fragments (PAGE).
 
 
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(Bottom Image) Running gel electrophoresis under ultraviolet transilluminator could clearly see the results.
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Students master their skills in using Gel Electrophoresis to identify RFPLS (Restriction Fragment Length Polymorphisms), which is a common method used in the diagnosis of Sickle Cell Anemia. They prepared agarose gels, loaded DNA samples with micropipettes, and ran the gels to determine which samples were normal, carriers, or affected by SCA.
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